st13 knockdown (Santa Cruz Biotechnology)
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St13 Knockdown, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/st13 knockdown/product/Santa Cruz Biotechnology
Average 85 stars, based on 2 article reviews
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1) Product Images from "ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines"
Article Title: ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines
Journal: Journal of Zhejiang University SCIENCE B
doi: 10.1631/jzus.b1200037
Figure Legend Snippet: Fig. 1 ST13 expression in colorectal cancer cell lines (a) qRT-PCR shows relative transcript levels of ST13 in six colorectal cancer cell lines, including RKO, HT29, SW480, SW620, LOVO, and LS174T, and HEK293. The relative quantity was normalized to HEK293 cells, and β-actin was used as an internal control. (b) qRT-PCR shows relative transcript levels of ST13 in ST13-lentivirus-infected Lenti- ST13, control lentivirus-infected Lenti-Mock, and SW620. The relative quantity was normalized to Lenti-Mock cells, and β-actin was used as an internal control. (c) qRT-PCR shows relative transcript levels of ST13 in SW620, control- shRNA-lentivirus-infected shRNA-Mock, and ST13-shRNA- lentivirus-infected shRNA-ST13. The relative quantity was normalized to shRNA-Mock cells, and β-actin was used as an internal control. (d) Western blot results show that ST13 was expressed in SW620 cells with different groups, including Lenti-ST13, Lenti-Mock, SW620, shRNA-Mock, and shRNA-ST13. β-actin was served as loading control. Error bars indicate SEM (n=3 experiments)
Techniques Used: Expressing, Quantitative RT-PCR, Control, Infection, shRNA, Western Blot
Figure Legend Snippet: Fig. 4 Effect of ST13 expression on SW620 cells mi- gration in vitro Lenti-ST13, Lenti-Mock, SW620, shRNA-Mock, and shRNA-ST13 cells were cultured in the top well of a transwell system and migration of SW620 cells was measured by counting the number of SW620 cells migrat- ing to the filter surface. (a) Quantitative measurement of invaded cells. Data are representatives of each group and expressed as mean±SEM of cells per six high power fields from three separated experiments. ** P<0.01, Lenti-ST13 vs. Lenti-Mock and SW620 (one way ANOVA and Dun- nett’s test). (b) Images of Lenti-ST13, Lenti-Mock, SW620, shRNA-Mock, and shRNA-ST13 cells on the filter surface
Techniques Used: Expressing, In Vitro, shRNA, Cell Culture, Migration
Figure Legend Snippet: Fig. 5 Effect of ST13 expression on the development and growth of inoculated SW620 cells BALB/c nude mice (n=4 per group) were inoculated with 5×106 shRNA-ST13 or shRNA-Mock cells and the devel- opment of solid SW620 tumors was monitored every 3‒4 d. The mice were sacrificed 32 d post-inoculation and the tumors were taken at the same time. (a) The dynamics of shRNA-ST13 and shRNA-Mock tumor growth. Data are expressed as mean±SEM for each group. (b) The images of individual tumors
Techniques Used: Expressing, shRNA